Tris Base CAS 77-86-1 Purity ≥99.50% (Titration) Biological Buffer Molecular Biology Grade
Shanghai Ruifu Chemical Co., Ltd. is the leading manufacturer and supplier of Tris Base (CAS: 77-86-1) with high quality, commercial production. Ruifu can supply a series of biological buffers, can provide worldwide delivery, small and bulk quantities available. Welcomed to order. Please contact: alvin@ruifuchem.com
Chemical Name | Tris Base |
Synonyms | Tris(hydroxymethyl)aminomethane; Trometamol; Trimethylolaminomethane; 2-Amino-2-(Hydroxymethyl)-1,3-Propanediol; THAM; Trizma Base |
CAS Number | 77-86-1 |
Stock Status | In Stock, Production Capacity 1000 Tons per Year |
Molecular Formula | C4H11NO3 |
Molecular Weight | 121.14 |
Melting Point | 167.0~172.0℃ |
Boiling Point | 219.0~220.0℃/10 mm Hg(lit.) |
Density | 1.353 g/cm3 |
Store Under Inert Gas | Store Under Inert Gas |
Sensitive | Hygroscopic |
Solubility in Water | Soluble in Water, 550 g/l 25℃ |
Solubility | Insoluble in Chloroform, Ether |
pKa | 8.1 (at 25℃) |
Stability | Stable. Incompatible with Bases, Strong Oxidizing Agents. Protect From Moisture |
COA & MSDS | Available |
Origin | China |
Product Categories | Biological Buffers |
Brand | Ruifu Chemical |
Item | Specifications | Results |
Appearance | White Crystalline Powder | White Crystalline Powder |
Purity | ≥99.50% (Titration) | 99.85% |
Melting Point | 168.0~171.0℃ | 168.4~170.8℃ |
Solubility | Clear, Colorless Solution (40% aq.) | Conforms |
Water Content (K.F) | ≤0.20% | 0.07% |
Insolubles Matter | ≤0.005% | 0.0045% |
Sulphated Ash | ≤0.05% | 0.0039% |
pH | 10.0~11.5 (10% aq.) | 10.48 |
Heavy Metals (as Pb) | ≤5ppm | 3ppm |
Iron (Fe) | ≤5ppm | 1ppm |
Sulfate (SO42-) | ≤10ppm | 3ppm |
Chloride (Cl) | ≤3ppm | 2ppm |
Arsenic (As) | ≤1ppm | <1ppm |
Ultraviolet Absorbance 260nm | <0.07 (20% aq.) | 0.050 |
Ultraviolet Absorbance 280nm | <0.07 (40% aq.) | 0.069 |
Ultraviolet Absorbance 400nm | <0.02 (40% aq.) | 0.019 |
DNase, RNase, Protease | Not Detected | Not Detected |
Infrared Spectrum | Conforms to Structure | Conforms |
Test Standard | Enterprise Standard | Conforms |
Package: Bottle, Aluminium foil bag, 25kg/Cardboard Drum, or according to customer's requirement.
Storage Condition: Keep the container tightly closed and store in a cool, dry and well-ventilated warehouse away from incompatible substances. Protect from light and moisture. Keep away from fire and heat source.
Shipping: Deliver to worldwide by air, by sea, by FedEx / DHL Express. Provide fast and reliable delivery.
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Hazard Symbols Xi - Irritant
Risk Codes
36/37/38 - Irritating to eyes, respiratory system and skin.
Safety Description
S26 - In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
S36 - Wear suitable protective clothing.
S37/39 - Wear suitable gloves and eye/face protection
S24/25 - Avoid contact with skin and eyes.
S23 - Do not breathe vapour.
WGK Germany 2
RTECS TY2900000
FLUKA BRAND F CODES 3
TSCA Yes
HS Code 2922199090
Hazard Class IRRITANT
Tris Base (CAS: 77-86-1), or Tris(hydroxymethyl)aminomethane, or known during medical use as Trometamol or THAM. It is extensively used as a zwitterionic biological buffer in biochemistry and molecular biology research, and enzymatic research. Tris base is widely used as a biological buffer or as a component of buffer formulations such as TAE and TBE buffers. Tris has a pKa of 8.06 and is very useful in the biology and biochemistry lab because it has buffering capabilities in the range of the typical physiological pH of most living organisms (pH 7.0~9.0). Tris has been reported to interfere with the activity of a number of enzymes, it should therefore be used carefully when studying proteins. Tris buffer, only nucleic acids and proteins are widely used as solvents. Tris is used for protein crystal growth under different pH conditions. Tris buffer having a low ionic strength can be used nematode (C. elegans) is formed lamin (lamin) intermediate fibers. Tris is a major component of protein electrophoresis buffer. Tris for the preparation of a surfactant, a vulcanization accelerator, and some drug intermediates.
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